Anticorps Recombinant de lapin anti-Angiopoietin 1

Angiopoietin 1 Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240764D10

Avis (2)

N° de cat : 81990-4-RR

Synonymes

ANGPT1, 240764D10, AGP1, AGPT, ANG 1

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Galerie de données de validation

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  WB analysis using 81990-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 81990-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 81990-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 81990-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 81990-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 81990-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung using 81990-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung using 81990-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 81990-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using 81990-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81990-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81990-4-RR against Human ANGPT1 were performed. The affinity constant is 6.37 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules K-562, cellules MG U-87, cellules Raji
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris, tissu pulmonaire humain
• Résultats positifs en FC (Intra): cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81990-4-RR cible Angiopoietin 1 dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Angiopoietin 1 Protéine recombinante Ag18829
• Nom complet: angiopoietin 1
• Masse moléculaire calculée: 498 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC152411
• Symbole du gène: Angiopoietin 1
• Identification du gène (NCBI): 284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-25-2025)

The antibody detected 3 bands around 55 kD, 75 kDa and 125 kDa at dilution of 1:4000 incubated at room temperature for 2 hours followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: A549

Angiopoietin 1 Antibody Western Blot validation (1:4000 dilution) in A549 (Cat no:81990-4-RR)

FH

Marine (Verified Customer) (07-01-2025)

It works well, it is specific with ANG1

• Applications: Western Blot
• Primary Antibody Dilution: 1/500 and 1/1000
• Cell Tissue Type: Tumoral cells, endothelial cells, mouse fibroblasts

Angiopoietin 1 Antibody Western Blot validation (1/500 and 1/1000 dilution) in Tumoral cells, endothelial cells, mouse fibroblasts (Cat no:81990-4-RR)

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