Anticorps Polyclonal de lapin anti-Angiopoietin 2

Angiopoietin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(30)

Avis (2)

N° de cat : 24613-1-AP

Synonymes

ANGPT2, AGPT 2, AGPT2, ANG 2, ANG2

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Galerie de données de validation

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  WB analysis using 24613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of PC-12 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IF/ICC: cellules PC-12, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Informations sur le produit

24613-1-AP cible Angiopoietin 2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiopoietin 2 Protéine recombinante Ag17938
• Nom complet: angiopoietin 2
• Masse moléculaire calculée: 496 aa, 57 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC126200
• Symbole du gène: Angiopoietin 2
• Identification du gène (NCBI): 285
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin-2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin-2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin-2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin-2 is expressed only at sites of vascular remodeling. Angiopoietin-2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.

Publications

Species	Application	Title
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	J Med Chem; Discovery of Novel Small-Molecule Antiangiogenesis Agents to Treat Diabetic Retinopathy.; Authors - Donghwa Kim
human	WB	Int J Mol Sci; MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2.; Authors - Ying-Hsien Huang
mouse	WB,IF	Exp Mol Med; Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization; Authors - Jung Min Ha
human	IHC	Front Oncol; MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis.; Authors - Hailin Li
human	IF	Biomed Pharmacother; Diosmetin inhibits tumor development and block tumor angiogenesis in skin cancer.; Authors - Jawun Choi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-25-2025)

The antibody detected several bands at dilution of 1:2000 incubated at room temperature for 2 hours followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549

Angiopoietin 2 Antibody Western Blot validation (1:2000 dilution) in A549 (Cat no:24613-1-AP)

FH

Marine (Verified Customer) (07-01-2025)

It is not specific, it binds at different molecular weight on the ladder

• Applications: Western Blot
• Primary Antibody Dilution: 1/500 and 1/1000
• Cell Tissue Type: Tumoral cells, endothelial cells, mouse fibroblasts

Angiopoietin 2 Antibody Western Blot validation (1/500 and 1/1000 dilution) in Tumoral cells, endothelial cells, mouse fibroblasts (Cat no:24613-1-AP)

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