Anticorps Recombinant de lapin anti-ANGPTL3

ANGPTL3 Recombinant Antibody for WB, IHC, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

250808F7

N° de cat : 86193-1-RR

Synonymes

ANG-5, Angiopoietin like protein 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3

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Galerie de données de validation

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  WB analysis using 86193-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86193-1-RR (ANGPTL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocellular cancer using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 86193-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of mouse kidney using 86193-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86193-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86193-1-RR against Human ANGPTL3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375, cellules HEK-293, cellules HuH-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: human hepatocellular cancer, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86193-1-RR cible ANGPTL3 dans les applications de WB, IHC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ANGPTL3 Protéine recombinante Ag2573
• Nom complet: angiopoietin-like 3
• Masse moléculaire calculée: 460 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC058287
• Symbole du gène: ANGPTL3
• Identification du gène (NCBI): 27329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.

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