Anticorps Polyclonal de lapin anti-ANK2

ANK2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31080-1-PBS

Synonymes

ANK 2, Ankyrin 2

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Galerie de données de validation

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  WB analysis using 31080-1-AP (same clone as 31080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31080-1-AP (ANK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (31080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31080-1-PBS cible ANK2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANK2 Protéine recombinante Ag34642
• Nom complet: ankyrin 2, neuronal
• Masse moléculaire calculée: 434 kDa
• Poids moléculaire observé: 250-260 kDa
• Numéro d’acquisition GenBank: NM_001148
• Symbole du gène: ANK2
• Identification du gène (NCBI): 287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

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