Anticorps Monoclonal anti-ANKRD1

ANKRD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1F11F1

N° de cat : 67775-1-Ig

Synonymes

CVARP, CARP, C193, C 193, Ankyrin repeat domain-containing protein 1

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Galerie de données de validation

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  WB analysis of mouse heart using 67775-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 67775-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 67775-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 67775-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANKRD1 antibody (67775-1-Ig, Clone: 1F11F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de lapin, tissu cardiaque de porc
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

67775-1-Ig cible ANKRD1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANKRD1 Protéine recombinante Ag29974
• Nom complet: ankyrin repeat domain 1 (cardiac muscle)
• Masse moléculaire calculée: 319 aa, 36 kDa
• Numéro d’acquisition GenBank: BC018667
• Symbole du gène: ANKRD1
• Identification du gène (NCBI): 27063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression

Publications

Species	Application	Title
human	IF	Life Sci Alliance; Patterning in stratified epithelia depends on cell-cell adhesion; Authors - Yosuke Mai
mouse	IF	J Pharm Anal; SUMO1 regulates post-infarct cardiac repair based on cellular heterogeneity; Authors - Zhihao Liu

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