Anticorps Monoclonal anti-ANKRD1

ANKRD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F11F1

N° de cat : 67775-1-PBS

Synonymes

CVARP, CARP, C193, C 193, Ankyrin repeat domain-containing protein 1

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Galerie de données de validation

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  WB analysis of mouse heart using 67775-1-Ig (same clone as 67775-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 67775-1-Ig (same clone as 67775-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 67775-1-Ig (same clone as 67775-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67775-1-Ig (same clone as 67775-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANKRD1 antibody (67775-1-Ig, Clone: 1F11F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67775-1-PBS cible ANKRD1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANKRD1 Protéine recombinante Ag29974
• Nom complet: ankyrin repeat domain 1 (cardiac muscle)
• Masse moléculaire calculée: 319 aa, 36 kDa
• Numéro d’acquisition GenBank: BC018667
• Symbole du gène: ANKRD1
• Identification du gène (NCBI): 27063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression

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