Anticorps Polyclonal de lapin anti-ANKRD11

• Phare
• Validé par KD/KO

ANKRD11 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55364-1-AP

Synonymes

ANCO 1, ANCO1, Ankyrin repeat domain-containing protein 11, Ankyrin repeat-containing cofactor 1, LZ16

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Galerie de données de validation

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  WB analysis of HeLa using 55364-1-AP

  WB result of ANKRD11 antibody (55364-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANKRD11 transfected HeLa cells.

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  WB analysis of BxPC-3 using 55364-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 55364-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

55364-1-AP cible ANKRD11 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ankyrin repeat domain 11
• Masse moléculaire calculée: 298 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: NM_013275
• Symbole du gène: ANKRD11
• Identification du gène (NCBI): 29123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANKRD11, also named as ANCO1, may recruit HDACs to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation. This antibody is specific to ANKRD11.

Publications

Species	Application	Title
human	WB	Am J Med Genet A; Two loss-of-function ANKRD11 variants in Chinese patients with short stature and a possible molecular pathway.; Authors - Tingting Zhang
mouse	IHC,IF	J Physiol Biochem; The effect of aspartame on accelerating caspase-dependent apoptosis of pancreatic islet via ZIPK/STAT3/caspase 3 signaling pathway; Authors - Haiying Hu

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