Anticorps Monoclonal anti-ANLN

• Phare
• Validé par KD/KO

ANLN Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B4A8

N° de cat : 66643-1-PBS

Synonymes

Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin

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Galerie de données de validation

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  WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

  WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66643-1-Ig (same clone as 66643-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66643-1-Ig (same clone as 66643-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66643-1-Ig (same clone as 66643-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANLN antibody (66643-1-Ig, Clone: 1B4A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66643-1-PBS cible ANLN dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANLN Protéine recombinante Ag19294
• Nom complet: anillin, actin binding protein
• Masse moléculaire calculée: 1124 aa, 124 kDa
• Poids moléculaire observé: 110 kDa~120 kDa
• Numéro d’acquisition GenBank: BC034692
• Symbole du gène: ANLN
• Identification du gène (NCBI): 54443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.

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