Anticorps Polyclonal de lapin anti-ANO2

• Phare
• Validé par KD/KO

ANO2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20647-1-PBS

Synonymes

anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B

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Galerie de données de validation

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  WB analysis using 20647-1-AP (same clone as 20647-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 20647-1-AP (same clone as 20647-1-PBS)

  IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using ANO2 antibody (20647-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20647-1-PBS cible ANO2 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: anoctamin 2
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: NM_020373
• Symbole du gène: ANO2
• Identification du gène (NCBI): 57101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

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