Anticorps Monoclonal anti-ANO2

ANO2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F12G1

N° de cat : 67638-1-PBS

Synonymes

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

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Galerie de données de validation

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  WB analysis using 67638-1-Ig (same clone as 67638-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67638-1-Ig (same clone as 67638-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67638-1-PBS cible ANO2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: anoctamin 2
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: NM_020373
• Symbole du gène: ANO2
• Identification du gène (NCBI): 57101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

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