Anticorps Recombinant de lapin anti-ANO4

• Phare
• Validé par KD/KO

ANO4 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

230308G1

Avis (1)

N° de cat : 82970-1-RR

Synonymes

Transmembrane protein 16D, TMEM16D, Anoctamin-4, anoctamin 4, 230308G1

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Galerie de données de validation

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  WB analysis using 82970-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82970-1-RR (ANO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82970-1-RR

  WB result of ANO4 antibody (82970-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANO4 transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules PC-3, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82970-1-RR cible ANO4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: anoctamin 4
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 100-111 kDa
• Numéro d’acquisition GenBank: NM_178826
• Symbole du gène: ANO4
• Identification du gène (NCBI): 121601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANO4, also named as TMEM16D, belongs to the anoctamin family. ANO4 is primarily expressed in the central nervous system and certain endocrine glands, and it has been linked to a variety of neurological disorders (PMID: 36836529). The antibody recognize the isoform1 and isoform2 of ANO4.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vivienne (Verified Customer) (09-08-2025)

Lysates were prepared without cooking. 1x10^6 cells were incubated with RIPA buffer and sonified. After this BCA assay was performed for analysing the protein concentration. 50 µl plus 25 µl 3X Lämmli buffer + mercaptoethanol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 in 5 % BSA
• Cell Tissue Type: A374, SH-4

ANO4 Antibody Western Blot validation (1:1000 in 5 % BSA dilution) in A374, SH-4 (Cat no:82970-1-RR)

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