Anticorps Polyclonal de lapin anti-ANO6

ANO6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 20784-1-AP

Synonymes

ANO6, anoctamin 6, TMEM16F, Transmembrane protein 16F

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Galerie de données de validation

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  WB analysis using 20784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20784-1-AP (ANO6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20784-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20784-1-AP (ANO6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 20784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20784-1-AP (ANO6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20784-1-AP (ANO6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20784-1-AP (ANO6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20784-1-AP (ANO6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 3 publications below

Informations sur le produit

20784-1-AP cible ANO6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANO6 Protéine recombinante Ag13937
• Nom complet: anoctamin 6
• Masse moléculaire calculée: 910 aa, 106 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC098410
• Symbole du gène: ANO6
• Identification du gène (NCBI): 196527
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Medicine (Baltimore); ANO6 is a reliable prognostic biomarker and correlates to macrophage polarization in breast cancer; Authors - Long-Huan Tang
human	IHC	Lab Med; Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer; Authors - Shiquan Xu
mouse		Life Sci; Benzbromarone improves blood hypercoagulability after TBI by reducing phosphatidylserine externalization through inhibition of TMEM16F expression; Authors - Kaiji Li
human	WB	Open Med (Wars); ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis; Authors - Hao Wang
mouse,human	WB,IHC	Neural Regen Res; TMEM16F may be a new therapeutic target for Alzheimer's disease; Authors - Zhi-Qiang Cui
pig	WB	Metabolites; Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease; Authors - Jianping Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christelle (Verified Customer) (04-22-2022)

nothing

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: lymphocytes B immortalized

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