Anticorps Monoclonal anti-ANP32A

ANP32A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D8C5

N° de cat : 67687-1-PBS

Synonymes

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

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Galerie de données de validation

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  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67687-1-PBS cible ANP32A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANP32A Protéine recombinante Ag8546
• Nom complet: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Masse moléculaire calculée: 249 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC007200
• Symbole du gène: ANP32A
• Identification du gène (NCBI): 8125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

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