Anticorps Monoclonal anti-ANP32B

• Phare
• Validé par KD/KO

ANP32B Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

5A1F12

N° de cat : 66160-1-Ig

Synonymes

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

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Galerie de données de validation

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  WB analysis using 66160-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 66160-1-Ig

  WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells.

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  WB analysis of fetal human brain using 66160-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66160-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66160-1-Ig (ANP32B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66160-1-Ig (ANP32B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66160-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32B antibody (66160-1-Ig, Clone: 5A1F12 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules HCT 116, cellules HEK-293, cellules HeLa, cellules U2OS, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:900-1:3600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

66160-1-Ig cible ANP32B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANP32B Protéine recombinante Ag1274
• Nom complet: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC013003
• Symbole du gène: ANP32B
• Identification du gène (NCBI): 10541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Publications

Species	Application	Title
human	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	J Virol; ANP32 proteins are essential for influenza virus replication in human cells.; Authors - Ecco Staller ; KO Validated
mouse,rat	WB	Orphanet J Rare Dis; HDAC5 inhibition attenuates ventricular remodeling and cardiac dysfunction; Authors - Chenxi Zhu
human	WB	Nat Commun; Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction; Authors - Liuke Sun

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