Anticorps Polyclonal de lapin anti-ANP32CP

• Phare
• Validé par KD/KO

ANP32CP Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31298-1-AP

Synonymes

ANP32C, PP32R1, Tumorigenic protein pp32r1

---

Galerie de données de validation

• 

  WB analysis of DU 145 using 31298-1-AP

  WB result of ANP32CP antibody (31298-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANP32CP transfected DU 145 cells.

• 

  WB analysis using 31298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31298-1-AP (ANP32CP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 31298-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ANP32CP antibody (31298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules DU 145, cellules LNCaP, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31298-1-AP cible ANP32CP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANP32CP Protéine recombinante Ag35293
• Nom complet: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: NM_012403.1
• Symbole du gène: ANP32C/PP32R1
• Identification du gène (NCBI): 23520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.

{{ptg:RelatedPrimaryAntibodies}}