Anticorps Recombinant de lapin anti-ANP32CP

ANP32CP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241562D12

N° de cat : 84553-1-RR

Synonymes

ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12

---

Galerie de données de validation

• 

  WB analysis using 84553-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84553-1-RR (ANP32CP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of LNCaP using 84553-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using ANP32CP antibody (84553-1-RR, Clone: 241562D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84553-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84553-1-RR against Human ANP32CP were performed. The affinity constant is 7.76 nM.

---

Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules BxPC-3, cellules DU 145, cellules LNCaP, cellules MCF-7
• Résultats positifs en IF/ICC: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84553-1-RR cible ANP32CP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ANP32CP Protéine recombinante Ag35293
• Nom complet: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: NM_012403.1
• Symbole du gène: ANP32C/PP32R1
• Identification du gène (NCBI): 23520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.

{{ptg:RelatedPrimaryAntibodies}}