Anticorps Polyclonal de lapin anti-CD13

CD13 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14553-1-AP

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Galerie de données de validation

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  WB analysis using 14553-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14553-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14553-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 14553-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 14553-1-AP (CD13 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 14553-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug CD13 antibody (14553-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules PC-3, cellules THP-1, tissu hépatique de souris, tissu hépatique humain, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal de souris,
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

14553-1-AP cible CD13 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, Lapin, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD13 Protéine recombinante Ag5976
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: ANPEP
• Identification du gène (NCBI): 290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Publications

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human		Anal Chem; Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray.; Authors - Weizhi Wang
human	WB,IF	Neuropathol Appl Neurobiol; Brain mural cell loss in the parietal cortex in Alzheimer's disease correlates with cognitive decline and TDP-43 pathology.; Authors - P Bourassa
human	WB	Front Oncol; YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma.; Authors - Qiang Xie
rat	WB	PLoS Negl Trop Dis; Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats.; Authors - Satish P RamachandraRao
mouse	IF	Exp Cell Res; siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.; Authors - Shion Osana