Anticorps Monoclonal anti-CD13

CD13 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

2D8D11

N° de cat : 66211-1-Ig

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

---

Galerie de données de validation

• 

  WB analysis of U-937 using 66211-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66211-1-Ig (CD13 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 66211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66211-1-Ig (CD13 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 66211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66211-1-Ig (CD13 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of U-937 using 66211-1-Ig

  1X10^6 U-937 cells were stained with 0.2 ug Anti-Human CD13 (66211-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules U-937
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal humain,
• Résultats positifs en cytométrie: cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:8000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66211-1-Ig cible CD13 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD13 Protéine recombinante Ag5976
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: ANPEP
• Identification du gène (NCBI): 290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Profiling the Surfaceome Identifies Therapeutic Targets for Cells with Hyperactive mTORC1 Signaling.; Authors - Junnian Wei
human	WB	Sci Rep; Proteomic Profiling of Small Extracellular Vesicles Secreted by Human Pancreatic Cancer Cells Implicated in Cellular Transformation.; Authors - Kelly A Servage
human	IF	Cancer Manag Res; Liver-enriched activator protein 1 as an isoform of CCAAT/enhancer-binding protein beta suppresses stem cell features of hepatocellular carcinoma.; Authors - Li-Hua Yang
		Biol Pharm Bull; Spautin-A41 Attenuates Cerulein-Induced Acute Pancreatitis through Inhibition of Dysregulated Autophagy.; Authors - Kai Dong
human	WB	Transl Cancer Res; Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer; Authors - Yunyan Shi
human	IHC	Theranostics; RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog.; Authors - Junxia Cao