Anticorps Polyclonal de lapin anti-ANT1/2

ANT1/2 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

Conjugaison

Non conjugué

N° de cat : 15997-1-PBS

Synonymes

SLC25A4, AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT

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Galerie de données de validation

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  WB analysis of human heart using 15997-1-AP (same clone as 15997-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15997-1-AP (same clone as 15997-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15997-1-AP (same clone as 15997-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15997-1-AP (same clone as 15997-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 15997-1-AP (same clone as 15997-1-PBS)

  IP result of anti-ANT1/2 (IP:15997-1-AP, 4ug; Detection:15997-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15997-1-PBS cible ANT1/2 dans les applications de et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANT1/2 Protéine recombinante Ag7787
• Nom complet: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC008664
• Symbole du gène: SLC25A4
• Identification du gène (NCBI): 291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.

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