Anticorps Polyclonal de lapin anti-ANTXR1

• Phare
• Validé par KD/KO

ANTXR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15091-1-AP

Synonymes

anthrax toxin receptor 1, ANTXR1, ATR, TEM8, Tumor endothelial marker 8

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Galerie de données de validation

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  WB analysis of mouse testis using 15091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15091-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 15091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, cellules PC-13, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15091-1-AP cible ANTXR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANTXR1 Protéine recombinante Ag4626
• Nom complet: anthrax toxin receptor 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 85 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC012074
• Symbole du gène: ANTXR1
• Identification du gène (NCBI): 84168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).

Publications

Species	Application	Title
human	WB,IHC	Am J Transl Res; MicroRNA-26b-3p/ANTXR1 signaling modulates proliferation, migration, and apoptosis of glioma.; Authors - Fei Geng
human	WB	Cancer Manag Res; KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1.; Authors - Yuanchun Li ; KD Validated
human	WB	Comput Math Methods Med; miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1.; Authors - Zhiqiang Dong