Anticorps Polyclonal de lapin anti-ANUBL1

ANUBL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17478-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

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  WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17478-1-PBS cible ANUBL1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANUBL1 Protéine recombinante Ag11505
• Nom complet: AN1, ubiquitin-like, homolog (Xenopus laevis)
• Masse moléculaire calculée: 653 aa, 72 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC048968
• Symbole du gène: ANUBL1
• Identification du gène (NCBI): 93550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).

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