Anticorps Polyclonal de lapin anti-Annexin A11

• Phare
• Validé par KD/KO

Annexin A11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10479-2-AP

Synonymes

56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50

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Galerie de données de validation

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  WB analysis using 10479-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10479-2-AP (Annexin A11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10479-2-AP

  HeLa (WT and ANXA11 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 10479-2-AP incubated at 1:2000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 10479-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10479-2-AP (Annexin A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10479-2-AP

  HeLa lysates prepared and IP of ANXA11 performed using 1.0 μg of 10479-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HeLa using 10479-2-AP

  IP result of anti-Annexin A11(IP:10479-2-AP, 4ug; Detection:10479-2-AP 1:1000) with HeLa cells lysate 2320 ug.

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  IHC staining of human ovary tumor using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10479-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10479-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10479-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10479-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu utérin de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

10479-2-AP cible Annexin A11 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin A11 Protéine recombinante Ag0729
• Nom complet: annexin A11
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC007564
• Symbole du gène: ANXA11
• Identification du gène (NCBI): 311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

Publications

Species	Application	Title
human	IHC,IF	Sci Transl Med; Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.; Authors - Bradley N Smith
human	IHC,IF	Ann Neurol; A Novel Multisystem Proteinopathy Caused by a Missense ANXA11 Variant.; Authors - Tauana Bernardes Leoni
mouse	WB	Oncotarget; ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.; Authors - Shuqing Liu ; KD Validated
mouse	WB	Biomed Pharmacother; Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.; Authors - Shuqing Liu ; KD Validated
human	WB,IHC	Neurobiol Aging; Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.; Authors - Elisa Teyssou
human	IHC	Ann Clin Transl Neurol; Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11; Authors - Mridul Johari