Anticorps Polyclonal de lapin anti-Annexin A4

Annexin A4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 10087-1-AP

Synonymes

ANXA4, ANX4, Annexin-4, Annexin IV, Annexin 4

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Galerie de données de validation

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  WB analysis using 10087-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10087-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10087-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10087-1-AP (Annexin A4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HuH-7, C6, cellules RAW 264.7
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

10087-1-AP cible Annexin A4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin A4 Protéine recombinante Ag0130
• Nom complet: annexin A4
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000182
• Symbole du gène: Annexin A4
• Identification du gène (NCBI): 307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.; Authors - Rogers Ronan S RS
human	WB	Aging (Albany NY); Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.; Authors - Ya Peng
mouse	WB	J Immunol; Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.; Authors - Kulik Liudmila L
human	WB	Mol Cell Proteomics; Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.; Authors - Hussein Kaddour
human	WB,IHC	Sci Rep; Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.; Authors - Kewu Jiang
human	WB	PLoS One; Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.; Authors - Qi Ying

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (09-30-2019)

The antibody is working very well on HeLa cells.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

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