Anticorps Polyclonal de lapin anti-Annexin A7

• Phare
• Validé par KD/KO

Annexin A7 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10154-2-AP

Synonymes

Annexin 7, annexin A7, Annexin VII, Annexin7, Annexin-7, ANX7, ANXA7, SNX, SYNEXIN

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Galerie de données de validation

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  WB analysis using 10154-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10154-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10154-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10154-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10154-2-AP

  IP Result of anti-Annexin VII (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:800) with mouse heart tissue lysate 3200ug.

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  IP experiment of U-87 MG using 10154-2-AP

  IP result of anti-Annexin A7 (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:8000) with U-87 MG cells lysate 1160 ug.

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  IHC staining of human pancreas using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10154-2-AP (Annexin VII antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10154-2-AP (Annexin VII antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of rat stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 10154-2-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Annexin A7 (10154-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules L02, cellules RAW 264.7, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules MG U-87, tissu cardiaque de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain, tissu d'estomac de rat, tissu d'estomac de souris, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

10154-2-AP cible Annexin A7 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin A7 Protéine recombinante Ag0206
• Nom complet: annexin A7
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 47 kDa, 51 kDa
• Numéro d’acquisition GenBank: BC002632
• Symbole du gène: ANXA7
• Identification du gène (NCBI): 310
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Am J Physiol Endocrinol Metab; Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma.; Authors - Eisenhofer Graeme G
human	WB,IF	J Proteome Res; Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.; Authors - Kun Meng ; KD Validated
human	IHC	Am J Transl Res; Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.; Authors - Hu-Fang Yuan
mouse	WB	Biofactors; Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo.; Authors - Ahmed Musa Hago Bakheet
human	WB	FEBS Lett; Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.; Authors - Willa Wen-You Yim