Anticorps Polyclonal de lapin anti-AOAH

AOAH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 12911-1-AP

Synonymes

Acyloxyacyl hydrolase, Acyloxyacyl hydrolase large subunit, Acyloxyacyl hydrolase small subunit, EC:3.1.1.77

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Galerie de données de validation

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  WB analysis of mouse colon using 12911-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12911-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de côlon de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12911-1-AP cible AOAH dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AOAH Protéine recombinante Ag3892
• Nom complet: acyloxyacyl hydrolase (neutrophil)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC025698
• Symbole du gène: AOAH
• Identification du gène (NCBI): 313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acyloxyacyl hydrolase (AOAH) is a highly conserved lipase responsible for hydrolyzing the secondary fatty acyl chains of gram-negative bacterial lipopolysaccharides (LPS), resulting in LPS detoxification and attenuation of host inflammation (PMID: 1577781, PMID: 35980963). AOAH has also been shown to have PLA2 function (PMID: 1577781). AOAH was highly expressed at the protein level in lesional psoriatic skin compared to healthy controls (PMID: 34913478). AOAH has been described to be differentially proteolytically processed into both a secreted and cytosolic form in phagocytic cells (PMID: 19755182). Expression of AOAH is primarily attributed to phagocytic cells such as neutrophils, macrophages, DC, and also NK cells (PMID: 33454018).

Publications

Species	Application	Title
	WB,IP	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han

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