Anticorps Polyclonal de lapin anti-VAP1/AOC3

VAP1/AOC3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 14365-1-AP

Synonymes

AOC3, VAP1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase

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Galerie de données de validation

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu adipeux de souris, tissu d'estomac de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de cancer du poumon humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

14365-1-AP cible VAP1/AOC3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAP1/AOC3 Protéine recombinante Ag5683
• Nom complet: amine oxidase, copper containing 3 (vascular adhesion protein 1)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC050549
• Symbole du gène: AOC3
• Identification du gène (NCBI): 8639
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences.; Authors - Fengchan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (10-21-2020)

Right size band and Clean background, but with relatively weak signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver lysate

VAP1/AOC3 Antibody Western Blot validation (1:1000 dilution) in mouse liver lysate (Cat no:14365-1-AP)

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