Anticorps Polyclonal de lapin anti-ACOX1

• Phare
• Validé par KD/KO

ACOX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(115)

N° de cat : 10957-1-AP

Synonymes

Peroxisomal acyl-CoA oxidase 1, A chain, Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1

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Galerie de données de validation

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  WB analysis using 10957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10957-1-AP

  WB result of ACOX1 antibody (10957-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells.

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  WB analysis of mouse kidney using 10957-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10957-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOX1 antibody (10957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu hépatique de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 97 publications below
• IHC: See 15 publications below
• IF: See 12 publications below

Informations sur le produit

10957-1-AP cible ACOX1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOX1 Protéine recombinante Ag1405
• Nom complet: acyl-Coenzyme A oxidase 1, palmitoyl
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 70-74kDa, 50 kDa, 22kDa
• Numéro d’acquisition GenBank: BC010425
• Symbole du gène: ACOX1
• Identification du gène (NCBI): 51
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
chicken	WB	Adv Sci (Weinh); α-Lipoic Acid Ameliorates Arsenic-Induced Lipid Disorders by Promoting Peroxisomal β-Oxidation and Reducing Lipophagy in Chicken Hepatocyte; Authors - Yangfei Zhao
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He ; KO Validated
mouse	WB	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park ; KO Validated
human	IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto

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