Anticorps Polyclonal de lapin anti-Aldehyde oxidase

• Phare
• Validé par KD/KO

Aldehyde oxidase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

Avis (1)

N° de cat : 19495-1-AP

Synonymes

EC:1.2.3.1, EC:1.17.3.-, Azaheterocycle hydroxylase, AOX1, AOX 1

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Galerie de données de validation

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  WB analysis using 19495-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19495-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19495-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 19495-1-AP

  IP result of anti-Aldehyde oxidase (IP:19495-1-AP, 4ug; Detection:19495-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19495-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldehyde oxidase antibody (19495-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu pancréatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

19495-1-AP cible Aldehyde oxidase dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: aldehyde oxidase 1
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 148 kDa
• Numéro d’acquisition GenBank: NM_001159
• Symbole du gène: AOX1
• Identification du gène (NCBI): 316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Integrated metabolomics and mass spectrometry imaging analysis reveal the efficacy and mechanism of Huangkui capsule on type 2 diabetic nephropathy; Authors - Jinwei Han
mouse	WB	EMBO Rep; Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.; Authors - Laura M Zidek
human	WB,IHC	Oncogene; Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.; Authors - Venkatrao Vantaku ; KD Validated
human	IF	Am J Obstet Gynecol; Evidence that fetal death is associated with placental aging.; Authors - Kaushik Maiti
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
human	WB	Front Immunol; Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.; Authors - Shun-Li Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MJ (Verified Customer) (05-27-2025)

Clean signal at 148kDA on rat brain lysate using 10% gel on 0.45um PVDF diluted in Hikari Signal Enhancer

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Brain lysate

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