Anticorps Polyclonal de lapin anti-AP1M1

• Phare
• Validé par KD/KO

AP1M1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12112-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Hacat cells using 12112-1-AP (same clone as 12112-1-PBS)

  WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12112-1-AP (same clone as 12112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12112-1-AP (same clone as 12112-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

• 

  IP experiment of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

  IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12112-1-PBS cible AP1M1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP1M1 Protéine recombinante Ag2757
• Nom complet: adaptor-related protein complex 1, mu 1 subunit
• Masse moléculaire calculée: 423 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017469
• Symbole du gène: AP1M1
• Identification du gène (NCBI): 8907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

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