Anticorps Monoclonal anti-AP1M1

AP1M1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E9A11

N° de cat : 68726-1-Ig

Synonymes

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

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Galerie de données de validation

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  WB analysis using 68726-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68726-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68726-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 68726-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68726-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP1M1 antibody (68726-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules A375, cellules HEK-293, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68726-1-Ig cible AP1M1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AP1M1 Protéine recombinante Ag25303
• Nom complet: adaptor-related protein complex 1, mu 1 subunit
• Masse moléculaire calculée: 423 aa, 49 kDa
• Poids moléculaire observé: 47-49 kDa
• Numéro d’acquisition GenBank: BC017469
• Symbole du gène: AP1M1
• Identification du gène (NCBI): 8907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

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