Anticorps Recombinant de lapin anti-AP1M1

• Phare
• Validé par KD/KO

AP1M1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240553H3

N° de cat : 83613-6-PBS

Synonymes

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

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Galerie de données de validation

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  WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83613-6-RR (same clone as 83613-6-PBS)

  WB result of AP1M1 antibody (83613-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1M1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 83613-6-RR (same clone as 83613-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83613-6-RR (AP1M1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83613-6-RR (same clone as 83613-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83613-6-RR (same clone as 83613-6-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83613-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83613-6-PBS cible AP1M1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AP1M1 Protéine recombinante Ag2757
• Nom complet: adaptor-related protein complex 1, mu 1 subunit
• Masse moléculaire calculée: 423 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017469
• Symbole du gène: AP1M1
• Identification du gène (NCBI): 8907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

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