Anticorps Polyclonal de lapin anti-AP2A1

AP2A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30030-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

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  WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30030-1-AP (same clone as 30030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30030-1-PBS cible AP2A1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP2A1 Protéine recombinante Ag32232
• Nom complet: adaptor-related protein complex 2, alpha 1 subunit
• Masse moléculaire calculée: 977 aa, 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC014214
• Symbole du gène: AP2A1
• Identification du gène (NCBI): 160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

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