Anticorps Polyclonal de lapin anti-AP3B1

• Phare
• Validé par KD/KO

AP3B1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 13384-1-AP

Synonymes

ADTB3, ADTB3A, AP 3 complex subunit beta 1, AP3B1, Beta 3A adaptin, HPS, HPS2, PE

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Galerie de données de validation

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  WB analysis using 13384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13384-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 13384-1-AP

  IP Result of anti-AP3B1 (IP:13384-1-AP, 3ug; Detection:13384-1-AP 1:500) with COLO 320 cells lysate 2500ug.

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  IHC staining of rat brain using 13384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules COLO 320, cellules HeLa, cellules HepG2, cellules SKOV-3, tissu de thymus de souris
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IF: See 2 publications below

Informations sur le produit

13384-1-AP cible AP3B1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP3B1 Protéine recombinante Ag4225
• Nom complet: adaptor-related protein complex 3, beta 1 subunit
• Masse moléculaire calculée: 1094 aa, 121 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC038444
• Symbole du gène: AP3B1
• Identification du gène (NCBI): 8546
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model.; Authors - Maria B Bagh
human	WB	Cell Rep; Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells.; Authors - Alexandros Strikoudis ; KO Validated
	WB	EMBO Rep; BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.; Authors - Ashari R Kannangara
human	WB	J Neurosci; Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.; Authors - Gokhale Avanti A
human	WB,IF	J Virol; Matrix Proteins of Nipah and Hendra Viruses Interact with Beta Subunits of AP-3 Complexes.; Authors - Weina Sun ; KD Validated
human	WB,IF	J Biol Chem; BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.; Authors - Bultema Jarred J JJ ; KD Validated

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