Anticorps Polyclonal de lapin anti-AP3D1

AP3D1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16454-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 16454-1-AP (same clone as 16454-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16454-1-AP (same clone as 16454-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

16454-1-PBS cible AP3D1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP3D1 Protéine recombinante Ag9390
• Nom complet: adaptor-related protein complex 3, delta 1 subunit
• Masse moléculaire calculée: 1153 aa, 130 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC010065
• Symbole du gène: AP3D1
• Identification du gène (NCBI): 8943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

{{ptg:RelatedPrimaryAntibodies}}