Anticorps Polyclonal de lapin anti-AP3M1

• Phare
• Validé par KD/KO

AP3M1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12114-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12114-1-AP (same clone as 12114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

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  WB analysis using 12114-1-AP (same clone as 12114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12114-1-AP (same clone as 12114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP3M1 antibody (12114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12114-1-PBS cible AP3M1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP3M1 Protéine recombinante Ag2759
• Nom complet: adaptor-related protein complex 3, mu 1 subunit
• Masse moléculaire calculée: 418 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC026232
• Symbole du gène: AP3M1
• Identification du gène (NCBI): 26985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

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