Anticorps Recombinant de lapin anti-AP4B1

AP4B1 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

242121D4

N° de cat : 84710-3-RR

Synonymes

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

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Galerie de données de validation

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  WB analysis using 84710-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 84710-3-RR

  IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug.

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  Affinity and Kinetic Characterization of 84710-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules HeLa, cellules HuH-7, cellules MCF-7, cellules NIH/3T3, cellules SH-SY5Y
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84710-3-RR cible AP4B1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AP4B1 Protéine recombinante Ag9348
• Nom complet: adaptor-related protein complex 4, beta 1 subunit
• Masse moléculaire calculée: 739 aa, 83 kDa
• Poids moléculaire observé: ~75 kDa
• Numéro d’acquisition GenBank: BC014146
• Symbole du gène: AP4B1
• Identification du gène (NCBI): 10717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.

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