Anticorps Polyclonal de lapin anti-AP50

• Phare
• Validé par KD/KO

AP50 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27355-1-PBS

Synonymes

AP2M1, Adaptin mu2, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu

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Galerie de données de validation

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  WB analysis using 27355-1-AP (same clone as 27355-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 27355-1-AP (same clone as 27355-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 27355-1-AP (same clone as 27355-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 27355-1-AP (same clone as 27355-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 27355-1-AP (same clone as 27355-1-PBS)

  IP result of anti-AP50 (IP:27355-1-AP, 4ug; Detection:27355-1-AP 1:1000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27355-1-PBS cible AP50 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP50 Protéine recombinante Ag26059
• Nom complet: adaptor-related protein complex 2, mu 1 subunit
• Masse moléculaire calculée: 433 aa, 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC013796
• Symbole du gène: AP50
• Identification du gène (NCBI): 1173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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