Anticorps Monoclonal anti-AP5Z1/SPG48

AP5Z1/SPG48 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G1D7

N° de cat : 66533-1-PBS

Synonymes

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

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Galerie de données de validation

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  WB analysis using 66533-1-Ig (same clone as 66533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66533-1-Ig (same clone as 66533-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66533-1-PBS cible AP5Z1/SPG48 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AP5Z1/SPG48 Protéine recombinante Ag26961
• Nom complet: KIAA0415
• Masse moléculaire calculée: 807 aa, 88 kDa
• Poids moléculaire observé: 88 kDa, 160 kDa
• Numéro d’acquisition GenBank: BC037399
• Symbole du gène: AP5Z1/SPG48
• Identification du gène (NCBI): 9907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.

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