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Anticorps Polyclonal de lapin anti-APAF1

APAF1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29022-1-PBS

Synonymes

APAF 1, APAF-1, Apoptotic protease-activating factor 1, CED4, KIAA0413

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using APAF1 Polyclonal antibody (29022-1-AP)

    WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 29022-1-AP (APAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29022-1-PBS in a different storage buffer formulation.

Informations sur le produit

29022-1-PBS cible APAF1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène APAF1 Protéine recombinante Ag30606
Nom complet apoptotic peptidase activating factor 1
Masse moléculaire calculée 1248 aa, 142 kDa
Poids moléculaire observé130-140 kDa
Numéro d’acquisition GenBankBC136532
Symbole du gène APAF1
Identification du gène (NCBI) 317
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

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