Anticorps Monoclonal anti-APBB1

APBB1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B9G10

N° de cat : 67077-1-PBS

Synonymes

APBB1, FE65, Protein Fe65, RIR

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Galerie de données de validation

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  WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67077-1-PBS cible APBB1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APBB1 Protéine recombinante Ag27436
• Nom complet: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
• Masse moléculaire calculée: 708 aa, 77 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC010854
• Symbole du gène: APBB1
• Identification du gène (NCBI): 322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).

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