Anticorps Polyclonal de lapin anti-APC
APC Polyclonal Antibody for IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (1)
Applications
WB, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 19782-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IHC | tissu de cancer du sein humain, tissu de cancer de l'endomètre humain, tissu de cancer du côlon humain, tissu de côlon humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunohistochimie (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 6 publications below |
Informations sur le produit
19782-1-AP cible APC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | adenomatous polyposis coli |
| Masse moléculaire calculée | 312 kDa |
| Numéro d’acquisition GenBank | NM_000038 |
| Symbole du gène | APC |
| Identification du gène (NCBI) | 324 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
APC, also named as DP2.5, belongs to the adenomatous polyposis coli (APC) family. APC is a tumor suppressor that regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division, and associates with other proteins involved in cell attachment and signaling. APC promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. It plays a critical role in several cellular processes. APC regulates beta-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. APC activity is correlated with its phosphorylation state. Defects in APC are a cause of familial adenomatous polyposis (FAP) which includes also Gardner syndrome (GS). Defects in APC are a cause of hereditary desmoid disease (HDD) which also known as familial infiltrative fibromatosis (FIF). Defects in APC are a cause of medulloblastoma (MDB) which is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) which also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1).
Protocole
| Product Specific Protocols | |
|---|---|
| IHC protocol for APC antibody 19782-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p. | ||
World J Gastroenterol MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation. | ||
Cancer Med MiR-26b suppresses hepatocellular carcinoma development by negatively regulating ZNRD1 and Wnt/β-catenin signaling. | ||
J Bone Oncol BAIAP2L2 promotes the proliferation, migration and invasion of osteosarcoma associated with the Wnt/β-catenin pathway | ||
Evid Based Complement Alternat Med Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Brianna (Verified Customer) (12-13-2018) |
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