Anticorps Recombinant de lapin anti-APCDD1

APCDD1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240085C6

N° de cat : 83246-6-RR

Synonymes

Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein

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Galerie de données de validation

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  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83246-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83246-6-RR against Human APCDD1 were performed. The affinity constant is 0.634 nM.

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules HEK-293T, cellules HeLa, cellules MCF-7, cellules Raji, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83246-6-RR cible APCDD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APCDD1 Protéine recombinante Ag10303
• Nom complet: adenomatosis polyposis coli down-regulated 1
• Masse moléculaire calculée: 514 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC053324
• Symbole du gène: APCDD1
• Identification du gène (NCBI): 147495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.

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