Anticorps Polyclonal de lapin anti-APEX1

APEX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 10323-1-AP

Synonymes

AP endonuclease 1, APE, APE1, APE1,APEX1, APEN, APEX, APEX nuclease, APEX1, APX, HAP1, Protein REF 1, REF1

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Galerie de données de validation

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  WB analysis of HeLa using 10323-1-AP

  WB result of APEX1 antibody (10323-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.

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  WB analysis of HepG2 using 10323-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10323-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10323-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10323-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10323-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10323-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10323-1-AP

  IP result of anti-APEX1 (IP:10323-1-AP, 4ug; Detection:10323-1-AP 1:500) with HepG2 cells lysate 2200 ug.

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  IHC staining of human cervical cancer using 10323-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10323-1-AP (APEX1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10323-1-AP

  Immunofluorescent analysis of HepG2 cells using 10323-1-AP (APEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules K-562, cellules Raji, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10323-1-AP cible APEX1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APEX1 Protéine recombinante Ag0397
• Nom complet: APEX nuclease (multifunctional DNA repair enzyme) 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004979
• Symbole du gène: APEX1
• Identification du gène (NCBI): 328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10323-1-AP is a rabbit polyclonal antibody raised against a fusion protein corresponding to an internal region of human APEX1.