Anticorps Recombinant de lapin anti-APEX1

APEX1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251693G12

N° de cat : 86719-1-PBS

Synonymes

APE, APE1, APE-1, APEN, APEX

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Galerie de données de validation

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  WB analysis using 86719-1-RR (same clone as 86719-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86719-1-RR (APEX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86719-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02621-2

  Plasma of six individual healthy human donors was measured. The APEX1 concentration of detected samples was determined to be 0.22 ng/mL with a range of 0.11-0.41 ng/mL.

• 

  Sandwich ELISA sample test of MP02621-2

  The mean APEX1 concentration was determined to be 141.21 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 137.64 ng/mL in HCT 116 cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02621-2

  Sandwich ELISA standard curve of MP02621-2, Human APEX1 Recombinant Matched Antibody Pair - PBS only. 86719-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0251. 86719-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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Informations sur le produit

86719-1-PBS cible APEX1 dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APEX1 Protéine recombinante Ag0251
• Nom complet: APEX nuclease (multifunctional DNA repair enzyme) 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002338
• Symbole du gène: APEX1
• Identification du gène (NCBI): 328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.

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