Anticorps Polyclonal de lapin anti-APH1A

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11643-1-PBS

Synonymes

CGI-78, CGI 78, Aph-1alpha, APH-1a, Aph 1alpha

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Galerie de données de validation

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  WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11643-1-AP (same clone as 11643-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11643-1-PBS cible APH1A dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APH1A Protéine recombinante Ag2238
• Nom complet: anterior pharynx defective 1 homolog A (C. elegans)
• Masse moléculaire calculée: 265 aa, 29 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC015568
• Symbole du gène: APH1A
• Identification du gène (NCBI): 51107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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