Anticorps Polyclonal de lapin anti-API5

API5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 25689-1-AP

Synonymes

AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL

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Galerie de données de validation

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  WB analysis using 25689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25689-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25689-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 25689-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 25689-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using API5 antibody (25689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 25689-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu pulmonaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

25689-1-AP cible API5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: API5 Protéine recombinante Ag22524
• Nom complet: apoptosis inhibitor 5
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC017709
• Symbole du gène: API5
• Identification du gène (NCBI): 8539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

Publications

Species	Application	Title
mouse	IHC	Sci Rep; microRNA regulatory circuits in a mouse model of inherited retinal degeneration.; Authors - Arpad Palfi
	WB	Gene; Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.; Authors - Shuang Zhang
human	WB	Oncol Lett; Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway.; Authors - Qiang Li