Anticorps Polyclonal de lapin anti-Apelin

Apelin Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 11497-1-AP

Synonymes

APLN, APEL, APJ endogenous ligand

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Galerie de données de validation

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  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 11497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 11497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris, tissu placentaire de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

11497-1-AP cible Apelin dans les applications de IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Apelin Protéine recombinante Ag2047
• Nom complet: apelin
• Masse moléculaire calculée: 77 aa, 9 kDa
• Numéro d’acquisition GenBank: BC021104
• Symbole du gène: Apelin
• Identification du gène (NCBI): 8862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Apelin, isolated from bovine stomach tissue extractsis, is recognized as the endogenous ligand of the angiotensin-like-receptor 1 (APJ), and the human orphan G-protein-coupled receptor. Apelin is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin has been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Apelin is also known to be involved in the maintenance of INS sensitivity and play an important role in liver disease.

Publications

Species	Application	Title
mouse		Sci Adv; Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.; Authors - Jun Seok Son
rat	IHC	Free Radic Biol Med; Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway; Authors - Liang Chen
mouse		EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat		Front Pharmacol; Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway.; Authors - Huaiqing Luo
mouse	IHC	Mol Med Rep; Apelin/APJ alleviates diabetic nephropathy by improving glomerular endothelial cells dysfunction via SIRT3‑KLF15; Authors - Mingcong Huang
	IF	J Cardiovasc Transl Res; Mesenchymal Stem Cell-based Apelin Gene Therapy Improves Pulmonary Artery Remodeling in Monocrotaline-induced Pulmonary Hypertension Through PI3K/AKT/eNOS and ERK1/2 Signaling Pathways; Authors - Li Wang

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