Anticorps Recombinant de lapin anti-APLP2

APLP2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241655G7

N° de cat : 84703-3-RR

Synonymes

241655G7, A4, Amyloid beta, Amyloid beta precursor like protein 2, Amyloid like protein 2

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Galerie de données de validation

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  WB analysis using 84703-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84703-3-RR (APLP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 84703-3-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using APLP2 antibody (84703-3-RR, Clone: 241655G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84703-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84703-3-RR against Human APLP2 were performed. The affinity constant is 0.675 nM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HEK-293, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84703-3-RR cible APLP2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APLP2 Protéine recombinante Ag6842
• Nom complet: amyloid beta (A4) precursor-like protein 2
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 95-120 kDa
• Numéro d’acquisition GenBank: BC000373
• Symbole du gène: APLP2
• Identification du gène (NCBI): 334
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Amyloid precursor-like protein-2 (APLP2) is a member of the APP (amyloid precursor protein) family including APP, APLP1, and APLP2. APLP2 is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. APLP2 has been shown to regulate multiple cellular functions such as neurite outgrowth, axogenesis, corneal epithelial wound healing, cell adhesion, migration, and mitosis. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. APLP2 has been implicated in the pathogenesis of Alzheimer's disease.

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