Anticorps Recombinant de lapin anti-Apolipoprotein AI

Apolipoprotein AI Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240269F1

N° de cat : 83504-1-PBS

Synonymes

APOA1, apolipoprotein A I, 240269F1, APOA 1, ApoA I

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Galerie de données de validation

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  WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein AI antibody (83504-1-RR, Clone: 240269F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Apolipoprotein AI Recombinant antibody (83504-1-RR, Clone:240269F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83504-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein AI were performed. The affinity constant is 4.05 nM.

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Informations sur le produit

83504-1-PBS cible Apolipoprotein AI dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Apolipoprotein AI Protéine recombinante Eg0757
• Nom complet: apolipoprotein A-I
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC005380
• Symbole du gène: APOA1
• Identification du gène (NCBI): 335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.

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