Anticorps Monoclonal anti-APOA4

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A9A8

N° de cat : 66252-1-PBS

Synonymes

3A9A8, APOA 4, apolipoprotein A IV

---

Galerie de données de validation

• 

  WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66252-1-Ig (same clone as 66252-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66252-1-PBS cible APOA4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOA4 Protéine recombinante Ag12454
• Nom complet: apolipoprotein A-IV
• Masse moléculaire calculée: 396 aa, 45 kDa
• Poids moléculaire observé: 45-46 kDa
• Numéro d’acquisition GenBank: BC074764
• Symbole du gène: APOA4
• Identification du gène (NCBI): 337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

{{ptg:RelatedPrimaryAntibodies}}